The retailer has opened two hubs in Wembley and Southwark, London, as well as a hub in Manchester.
The move is part of Amazon’s five-year, £300m ($364m) investment in the electrification and decarbonisation of its UK transportation network.
The launch marks the retailer’s first e-cargo bike deliveries in Manchester, while Amazon expects its new delivery hubs in London to more than triple its e-cargo bike fleet in the city.
Amazon UK country manager John Boumphrey said: “With more than €1bn committed to electrifying and decarbonising our European transportation network over the next five years, including more than £300m in the UK alone, we remain laser-focused on reaching net carbon zero by 2040.
“These new hubs will not only bring our customers more electric-powered deliveries, but also support local authorities looking for ways to reduce congestion and find alternative transportation methods.
“We look forward to expanding our e-cargo bike fleet further in the coming months.”
The new hubs will complement Amazon’s first UK micromobility hub, which was launched in Central London earlier this year.
The retailer claims to have completed more than five million deliveries using its e-cargo bikes and electric van fleet within London’s Ultra Low Emission Zone so far this year.
In the UK to date, Amazon has deployed more than 1,000 electric delivery vans and five fully electric Heavy Goods Vehicles to replace traditional diesel trucks.
The company has also announced micromobility expansion plans across France and Italy.
In Europe, it operates hubs in more than 20 cities to facilitate e-cargo bike and on-foot deliveries.
Amazon has pledged to reach net-zero carbon by 2040 as a co-founder of and the first signatory to The Climate Pledge.
The retailer also aims to power all its operations with renewable energy by 2025.